FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23140962
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1463384763.237850699551736Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA7848323.391527111102814Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC3232261.3967699354936065No Hit
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2581891.1157228467857128Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1798900.7773661267841847No Hit
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT1759570.7603702905695969Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC1077100.46545169556909516Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA826660.3572280184376086Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA674010.2912627400710481Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA655610.2833114716665625Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC582680.2517959279307403TruSeq Adapter, Index 12 (100% over 50bp)
TCACCGGGTGTAAATCAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA580540.25087116084456645Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAGATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA522150.22563884768489745Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCNCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA483490.2089325413524295Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGCGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA458530.19814647290808396Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG431590.18650477884195132Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAACCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA420290.18162166291963142Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG418080.18066664644278832Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT410010.17717932383277757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC408920.17670829760664228No Hit
TCACCGGGTGTGAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA401310.17341975670674364Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT400100.17289687438231824Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TNACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA391670.1692539834774371Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA382550.1653129200073878Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT381360.16479868036601072No Hit
TCACCGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG367020.15860187662034103Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC365300.1578586058781826No Hit
TCACGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG353410.15272053080593626Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA332560.14371053372802736Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTAAACTCCA326810.14122576235162565Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCACCGGGTGTAAATCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC310000.13396158724948426Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGT296810.12826173777909491Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAATCAGCTTGGGATCGGAAGAGCACACGTCTGAACTCCA296250.128019742653741Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT295290.12760489386742002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAGTCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA292460.12638195421607795Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG283570.1225402816010847Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGCAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA274830.11876342910895406Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACTGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA267340.11552674430734557Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG266350.11509893149645205Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT262090.11325804000715269No Hit
TCACCGGGGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG258330.11163321559406215Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC239540.10351341487013375Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCACCGGGTGTAAATCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCAG233900.10107617825049796Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA231480.10003041360164716Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)