FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14178579
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA631464844.53653641877652Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC5465493.8547515939361765No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA3024942.1334578027882767Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2561911.8068877001002708No Hit
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1133260.7992761474898155Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC587660.4144703076380221TruSeq Adapter, Index 2 (100% over 50bp)
TCACCGGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC525250.37045320267990184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT507180.3577086251026989Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA426510.3008129376011517Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT399300.28162201585927615No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT345760.2438608269559312Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA339070.2391424415662529Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC288170.20324321640412626No Hit
ACTTTGAAGACTGAAGTGGGGAAAGGTTCCATGTGAGATCGGAAGAGCACA265440.18721199070795458No Hit
TGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCATGTGAGATCGGAAGA251990.1777258496778838No Hit
TCACCGGGTGTAGATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA244220.17224575184861615Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA232340.16386691501313355Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAACCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA218450.1540704466928597Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCNCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA217660.15351326814908603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGCGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA212580.14993039852583254Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGATGCTGACGCAATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC207730.14650974544063972Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT191730.13522511670598303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTGAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA187340.13212889669691158Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA183710.12956869655273634Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG178940.12620446661121681Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGCAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA177390.12511126820254695Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG174470.12305182345847211Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT172730.1218246200835782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA171000.12060446960164345Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA165930.11702865287134909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA164320.11589313710492427Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC161190.11368558160870705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCACCGGGTGTAAGTCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA159040.11216920962248755Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCATCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA153810.10848054660484664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCCGACCGACCTTGAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA153710.10841001767525504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGAGGAAAAGAAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA153420.10820548377943941Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCACCGGGTGTAAATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA152730.10771883416525732Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG142850.10075057592160681Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)