FastQCFastQC Report
Wed 17 Jul 2024
EGAF00001839525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19854219
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC975351449.125649314133184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT10686415.382437858673765No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG10538945.308161454248087No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCC4916632.476365350860691Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2645431.3324271279570352No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1780620.8968471638194382No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1768230.8906066765960424No Hit
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1596840.8042824550288279Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTC851390.42882069549046475Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG748370.37693247969109234Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA739230.3723289241445357Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC676660.3408142118307449No Hit
CCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC651560.32817206257269554Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG645400.325069447455979No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT585960.2951312262648055No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG580960.2926128698388992No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT565520.28483618519570075No Hit
TCGCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC525460.2646591135113398Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG429290.21622104601545897No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT384870.19384796752770783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAGATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC378510.1906446181539551Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCGGCTTGAGATCGGAAGAGCACACGTCTGAACTCC352760.17767508256053788Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC336670.16957101158197155No Hit
TCACCGGGCGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC335710.16908748714819757Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCNCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC333650.16804992430072418Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAACCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC331600.16701739816610264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT318120.16022790924185937No Hit
TCACCGGGTGTAAATCAGCTTTAGATCGGAAGAGCACACGTCTGAACTCC297040.14961051855023863Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA290850.14649279329496667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTGAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC284620.1433549211882875Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGCAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC278810.14042859102138444Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT273640.13782461047699734No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG273170.13758788497296215TruSeq Adapter, Index 3 (100% over 49bp)
TCACCGGGTGTAAATCAGCTCGAGATCGGAAGAGCACACGTCTGAACTCC268280.13512493238842585Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC255180.12852683855255148Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCC237640.11969244421047236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAGTCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC234390.11805551253363326Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCATCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC233440.1175770248127111Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA231000.11634806687686884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC228730.11520473305950742No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT225400.11352750767985385No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC224650.11314975421596792No Hit
TCACGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA212490.10702511138816388Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTAAACTCC207440.10448157139799857Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCACCGGGTGTAAATCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA204670.10308640193804651Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG204350.10292522712678852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACTGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC202770.10212942649620213Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)