FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19854219
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA972119148.96284764462405Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC10651905.365056162622161No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT10510545.293857189748939No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA4900802.468392234416272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2638091.3287301807238048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1775770.894404358086309No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1763710.888330082387023No Hit
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1590760.801220133614926Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC849220.42772772880162147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT745630.3755524203696957Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC737710.3715633437910602Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT674970.33996300735878854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA649460.3271143528738149Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT644070.32439956464668795No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC584630.2944613434555144No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT579490.29187247304968283No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC563770.28395476044663354No Hit
TCGCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA523660.2637525051980136Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT428030.2155864201961306No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG382560.19268448685893913Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGGGTGTAGATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA377270.19002006576033034Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA351280.17692964905846964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT335610.16903712001967944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGCGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA334520.16848811831883187Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCNCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA332120.16727930723439688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAACCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA330670.16654898387088407Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC317350.15984008235226982No Hit
TCACCGGGTGTAAATCAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA295970.14907159027509467Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG289650.14588838775274918Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTGAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA283740.142911690457328Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGCAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA277920.1399803235775731Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC272820.13741160002314873TruSeq Adapter, Index 3 (100% over 50bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC272630.13731590247896427No Hit
TCACCGGGTGTAAATCAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA267360.1346615548060591Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA254190.12802820398022202Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA236850.11929454389517916Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAGTCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA233530.1176223552283774Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCATCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA232550.11712875736889979Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG230330.1160106071157974Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT228050.11486223658558414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC224820.11323537833444872No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT223960.11280222102919284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG211640.10659699079575984Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTAAACTCCA206490.10400308367707639Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA203850.1026733914841979Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCACCGGGTGTAAATCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG203600.10254747366290258Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACTGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA201970.10172648946805715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)