FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001839526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13895469
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA803274457.80836904461447Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC6802764.895667789262816No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA3268802.352421497971749Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3143432.262197843052293No Hit
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1303990.9384282027472409Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC710350.5112098051530323Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT690240.4967374616862519Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA547010.393660696159302Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT458850.3302155544372054No Hit
TCGCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA434520.31270624978545164Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT329860.23738673376191907Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAGATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA309580.22279204825688143Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC298610.21489738849404796No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC296200.21316301018698974No Hit
TCACCGGGTGTAAATCGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA289190.2081182002565009Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAACCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA275690.19840280310078054Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGCGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA275010.19791343494775168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC271900.19567529530669314No Hit
TCACCGGGTGTAAATCAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA271430.19533705555386435Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCNCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA270490.19466057604820677Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT267130.1922425216450053No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC248370.1787417178937969TruSeq Adapter, Index 4 (100% over 50bp)
TCACCGGGTGTGAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA238830.1718761705704212Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT234990.16911267982390518No Hit
TCACCGGGTGCAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA230470.1658598209243603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA221690.15954121447789923Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA209010.15041593774200784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG194940.14029033492860155Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAGTCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA194710.14012481334743002Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG190660.1372101942007139Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAATCAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCA190400.13702308284808523Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCATCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA188070.13534627726491275Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACTGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA168730.12142807126553268Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG156590.1126914104158701Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA155850.11215886271992691Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TNACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA155800.11212287976749831Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACAAGTCTGAACTCCA154630.11128087868066922Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCACCGGGGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG153160.11022297987926856Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGGGCACACGTCTGAACTCCA149870.10785530160946709Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCACCGGGTGTAAATCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG146710.10558117901597996Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGAAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA146650.10553799947306565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA145910.10500545177712245Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC141860.10209083263040634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)