FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14571152
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC157854310.833343856408883No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT14078569.661940250160042No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT3367162.310839939079628No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA2589501.7771415739812473Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC1168530.8019475742206245No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1086640.745747487913104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC831280.5704971027685388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT746070.5120185418421275No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC687840.47205601863188307TruSeq Adapter, Index 11 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC681130.46745102926659465No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC638510.4382014544903519No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT476640.3271120910687089No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT456240.31311182533817505No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT416010.2855024777725193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT410710.2818651538327237Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT391650.26878451340017595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC311450.2137442530281751No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG300880.20649019377465833No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG298170.2046303545526119No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG293200.20121950549963377Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC254720.17481116112164638No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC242450.16639041305725175No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT218860.15020089008748244No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA189180.12983187602462729Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT186970.12831518057048613Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT178050.1221934957510566No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC171420.1176434093886331TruSeq Adapter, Index 6 (97% over 36bp)
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA164100.11261978462650037Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT159320.10933933020532624No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC155480.10670398606781399Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG155140.10647064830563843Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA154410.10596965840449678Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG153050.10503630735579453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)