FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15871796
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT313342819.742113620915994No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC11338897.1440497345101965No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC8536995.378717065163892No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT5311643.346590392164819No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT5310393.345802831639217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT4984203.140287337362451No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT4854203.0583810426998936No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC4118572.5948985231413No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC3112421.9609753048741303No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT2566771.6171893842385574Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC2063841.3003191321259422No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT1007360.6346855768559525No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT996640.627931457788394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT995090.626954882736648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG884180.557076212421077No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT843520.5314584436443109No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA828870.5222282342842612Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT763630.481123875332067No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG632630.39858753224902843Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC595820.375395449891115No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC588650.37087800271626475No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC530190.3340453720549332No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGTC479730.30225312875745125No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT467500.294547636574966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC448600.28263972142787114No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG428070.2697048273553919No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGTAGATCGGAAGAGCACACG406870.25634780084119024No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC351870.22169513771472366No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT344040.21676185858235578No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT318910.20092874177566294No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT308200.19418092319230917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACGT290600.18309207099183986No Hit
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA273360.1722300362227438Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTTTTTCGGCGTGGACTCTGCGTTGGGGACTAGATCGGAAGAGCACACGT268000.16885297668896448No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC258060.16259029538938127No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT243730.1535617015238855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGGCGTGGACTCTGCGTTGGGGACTAGATCGGAAGAGCACACGTCTGAA239330.1507894884737682Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT238200.1500775337586244No Hit
TCGGCGTGGACTCTGCGTTGGGGACTAGATCGGAAGAGCACACGTCTGAAC233940.14739352748737447Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG231430.14581210595196661Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGNTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT230910.14548448077331638No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTCT221040.13926590286316684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT206880.13034441722915288TruSeq Adapter, Index 8 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA203140.12798803613655316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC196790.12398722866649747No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACGT193430.12187026597368061No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT192200.12109530641648872No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG191910.12091259237454917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC188390.11869482193445531No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT182390.11491453141156804Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACGT178830.11267155903465494No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC172690.1088030617329003Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT172110.10843763364902119Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC167530.10555201188321725TruSeq Adapter, Index 8 (100% over 50bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC163360.1029247099698106Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)