FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19251626
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT345421817.942474053879916No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC207072610.756109639777959No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT6691673.475898607213749Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC4811192.4991083870006614No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT4103012.131253744488907No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1970431.0235135463362939No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1447860.7520715393079005No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1326910.6892456772222773No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT1222820.6351775169536329No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1210390.6287209194693477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1180170.613023544089211No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1020910.5302980641739041No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC975130.506518254613922No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC917940.4768116729464826No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT714980.37138681169060733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG608690.31617589080527536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC605330.314430583681607TruSeq Adapter, Index 10 (100% over 50bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG548280.28479672314432036No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT538550.27974260459869726Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG442250.22972085578641513No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT398730.20711497304175763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT356070.18495580581089618No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT354010.18388576632436138Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC347840.18068084223119646No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT318140.1652535739059132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT309490.16076044693575492No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC306140.159020334178526No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA301630.15667767491431633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG268080.13925057550982967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT262150.1361703162112125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG261100.1356249077350661Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG249010.12934491870972353No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG247170.1283891552848575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT241830.1256153636061702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC236070.12262340853702436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC206390.10720652894462006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC205550.10677020216370295No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTCT199140.1034406132759903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)