FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13680691
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC3926102.8698111813211775No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1382571.0105995376987902TruSeq Adapter, Index 1 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1189380.8693859104046717No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT868860.6350994989946049No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT688640.5033663869756286Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT675440.49371775153755026Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC502810.36753260489546913No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC478190.3495364378889926No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC397910.2908551914519522No Hit
GACGCTGTGGCCGAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC220090.1608763767853539Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT211860.15486059878115804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC210990.15422466599092108No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA204210.14926877597045354Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT199620.14591368228403082No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT195660.14301909165260732Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT194610.142251586560942No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC152780.11167564562345571No Hit
GACGCTGTGGCCGAACGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT149220.10907343788409518Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT148930.10886146028734951Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGCTGTGGCCGAACGGTTAAGAGATCGGAAGAGCACACGTCTGAACTCC146450.10704868635655904Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)