FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17800113
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC186285410.465405472425934No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT17149899.634708498760654No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT4193542.3559063922796444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3787452.127767391139596No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT2970891.6690287303232287No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2594151.4573783885529266TruSeq Adapter, Index 1 (100% over 50bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC2075041.1657454084701597Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1500360.8428935254512148No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1231990.6921248196570438No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1135170.6377319065334023No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1132140.6360296701487232No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1026330.5765862272896807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT1001940.5628840670842932No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC972750.5464852947843646No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC847660.47621046001224826No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG760370.42717144548464386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT759560.42671639219369Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT639220.35911007980679677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA634100.35623369357261947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC632700.35544718171171164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT625050.35114945618603655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA590000.3314585699540222Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT562510.3160148477709102No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT555660.31216655759432543Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG547290.30746434025446917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT494050.2775544177725164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC477710.26837470076734904Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG463120.26017812358831655Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC437810.24595911273147536Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC435070.24441979666084143No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA390540.21940310154210818Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT353590.19864480635600462No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT347630.1952965130052826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT331770.1863864572095694Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT279660.1571113621582065Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC267040.15002151952630863No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC240470.13509464799465037No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG235880.13251601267924534Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA230040.1292351346308869Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG226390.12718458585066286No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT214870.12071271682376399No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA206480.11599926360018051Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG204180.11470713697154619Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC198090.11128581037659704No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC193430.10866784946814663Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT189630.10653303155996818Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG187100.10511169226847045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG181380.10189822952247551Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG181080.10172969126656668No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGTC180580.10144879417338531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)