FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17171798
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC12111957.053396505130098No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6397443.725550463614818No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT5361103.122037657326274No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT5000372.911966469673123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC2760091.6073389635727138No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2567901.495417078630904TruSeq Adapter, Index 10 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT2274311.324444883407084No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1372550.7993047670372084No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1321940.7698320234142051No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT1166380.6792416262991213No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1153140.6715313096508589No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1044340.6081716078887022Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG923110.537573293140299Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT896220.5219138962617659Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT694790.404611095471773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC602950.3511280531019524No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC541620.31541251533473663No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG539600.3142361679306966No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA479570.2792776854235066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT413030.2405281031141876No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC363100.21145135762719783No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT356970.20788155090107627Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT332570.1936722060205926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGTC321990.1875109409043829No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC287150.16722185993569222Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG280480.16333758410156002No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT274480.15984348290144107No Hit
TCCCTGGTGGTCTAGTGGTAGGATTCGGCGAGATCGGAAGAGCACACGTCT260260.15156246305715917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT249930.14554678549095443No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT240020.13977569500875794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACACG235030.13686976751065905No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT232950.1356584790946178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT220600.128466454124373Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG219450.1277967513943502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT216520.12609046530829213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA210490.12257889360217258Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT209370.12192666137815038No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT195540.11387275811187622No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG193020.11240523560782628Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT177390.10330310198151643Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACGT175740.10234222415148374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)