FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001839559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213376
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4995296.92503759682013No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT4409136.1124361186773015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA4405306.107126538253378Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2508643.477761314535663No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1791982.4842459342199823No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1029981.427875103141719Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC629120.872157502950075No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA609160.8444866869549015Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG587390.8143066436575607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC567550.7868021852735807No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT500210.6934478391255358No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT474440.6577225421217472No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC336110.4659538058185238Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT333380.4621691701638733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT291910.4046787523622781Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC253610.3515829481230425No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT247740.3434452883088307Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC232920.3229001233264424No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA202010.2800491753098688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA193510.26826551118366765Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT190750.26443928612621886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG188480.26129235464780987Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC149810.2076836144407279No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG141060.19555337195787384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT140700.19505429912429353Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG140200.1943611424109876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC138420.1918935045116184No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC124190.17216626445093117TruSeq Adapter, Index 11 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT120860.16754984074031354No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT120090.1664823794018224TruSeq Adapter, Index 11 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT114490.1587190242127958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT106510.14765624306843286No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT101150.14022560310179313Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT97730.13548441118278043No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAA96470.13373765626524944Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA87480.12127469856000853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT86210.11951408050821141Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT85460.1184743454382525Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT81240.11262410277795029Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACGT79680.11046145383243573No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT79070.10961580264220248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT78050.10820176294705836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG77130.1069263545945754Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC76550.10612229280714053No Hit
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG75100.10411213833855326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC72920.10108997506853934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)