FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20143842
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA855329542.46109059036504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4276142.122802591481804No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA3285021.6307812581135217Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2296081.1398421413353024No Hit
TCACCGGGTGTAAATCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1563350.7760932596671479Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTAAATCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT1448470.7190634239486192Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT839690.4168469947292081Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCC705380.35017153132952494Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC534780.2654806367126986TruSeq Adapter, Index 12 (100% over 50bp)
TCACCGGGTGTAAATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC532010.26410552664183923Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA453290.22502658628875266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT399460.1983037793882617TruSeq Adapter, Index 12 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT392380.19478905761870055No Hit
TCACCGGGTGTAAATCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC372150.1847462862347709Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA350530.1740134776672692Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAGATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA297770.14782185046924018Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCGGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA296010.14694813432313458Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA270080.13407571405693117Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGCGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA263010.1305659565836547Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGGTGTAAATCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT256910.12753773584999328Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCACCGGGTGTAAACCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA246830.12253372519502485Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG236520.11741553572550856Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCACCGGGTGTGAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA230580.11446674373240219Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC226840.1126100969219278No Hit
TCACGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG226100.11224273899686067Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC205480.1020063600578281No Hit
TCACCGGGTGTAAATCAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG201610.10008517739565272Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)