FastQCFastQC Report
Wed 17 Jul 2024
EGAF00001839591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18150356
Total Bases925.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT212799911.724282432807378No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8113204.469994968693727Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG6578903.6246671966103587No Hit
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC5598683.0846116737324603Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3565181.964247973979133No Hit
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAG2840951.5652310070392008Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1791040.9867795430568964Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1598270.8805722598498895Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGCCCTGGCGGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1538850.847834609965777Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAA1445790.7965628883532642Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1304170.7185368705715744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT1223540.6741134994817732No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG1179010.6495795454370151No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG1043080.5746884523917878Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG983900.5420830313190551TruSeq Adapter, Index 10 (100% over 49bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCAC954390.5258243970531487No Hit
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA951930.5244690517365058TruSeq Adapter, Index 10 (100% over 35bp)
CAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT914770.5039956241078687Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG913870.5034997660651945TruSeq Adapter, Index 10 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC845360.4657539499500726No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC841340.4635391173594612Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT760220.41884577911309284No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG620760.3420098206338212No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA572060.31517839099133926Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT570120.31410954143268593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC498850.2748430939866965No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC475880.2621876948308893No Hit
GCAGCGGAAGCGTGCTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAG443420.24430374809177297Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC436930.24072806065071123Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT403630.22238131307176565No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT397380.21893785444208366No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACA388740.2141776172324113No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCA376240.20729069997304736Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAAC348990.19227722034763395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGAAACTCTGGTGGAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT321020.1768670542880812Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT293790.16186459373028275Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGGGTGTGCCGTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC278110.15322564472013664Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCGGCGACTCTGGACGCGAGCAGATCGGAAGAGCACACGTCTGAACTCC273470.15066922103346073Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGAAGCGTGCTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA264650.14580981221525352Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACAGATCGGAAGAGCACACGTCTGA263110.14496134400889987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTG259260.14284017349301578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG257620.14193660994858723No Hit
ACGGCCCTGGCGGAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC255840.14095591293085383Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGAAACTCTGGTGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT252640.13919286211245666Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT246960.13606344690980166No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC245340.1351709024329881No Hit
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT242870.13381004758253778Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA232280.12797545128040463TruSeq Adapter, Index 10 (100% over 35bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC229190.12627300533388988TruSeq Adapter, Index 10 (100% over 37bp)
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT216520.11929242599979857Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGAAACTCTGGTGGAGGTAGATCGGAAGAGCACACGTCTGAACTCCAG206540.11379391125992239Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC190320.10485744742417173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA189540.10442770378718742TruSeq Adapter, Index 10 (100% over 35bp)
AGCTTGACTGGAGAAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT188910.10408060315731549Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC187060.10306133940292962Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)