FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18150356
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC212264311.694773369734456No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT8084644.454259740139532Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6570423.619995111941606No Hit
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5589053.079305992675846Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3560531.9616860407586496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT2836281.5626580547511024Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1789440.9858980176476979Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1597080.879916625326798Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1535990.8462588832968345Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1443740.7954334339227286Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1300300.7164046809880754Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC1221190.6728187590370128No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1177720.6488688155758487No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1041380.5737518316445143Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC983470.5418461213653329TruSeq Adapter, Index 10 (100% over 50bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACA953120.5251246862595973No Hit
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG951100.5240117604304841TruSeq Adapter, Index 7 (97% over 36bp)
CAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC913660.5033840658552372Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC913130.5030920605634401TruSeq Adapter, Index 10 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT844310.465175448900286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA839750.4626631014840701Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC759220.4182948257323437No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT620150.34167373907156423No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG571320.31477068548958487Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG568620.3132831113615622Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT498180.27447395522159457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT475230.2618295751334023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGCGGAAGCGTGCTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT442720.24391808072524856Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC436320.24039197908845422Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC403140.22211134591519857No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC396850.21864584915028662No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACAC388350.21396274541391913No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG375680.20698216607982783Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT348480.1919962341234519Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGAAACTCTGGTGGAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC320600.17663565386816654Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA293430.1616662505132131TruSeq Adapter, Index 7 (97% over 35bp)
AAGGGGTGTGCCGTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA277640.15296669663118453Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCGGCGACTCTGGACGCGAGCAGATCGGAAGAGCACACGTCTGAACTCCA272780.15028906320074384Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAAGCGTGCTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC264500.14572716920814116Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACAGATCGGAAGAGCACACGTCTGAA262590.14467484825091034Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA258860.14261979214071616Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT257240.14172724766390257No Hit
ACGGCCCTGGCGGAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA255450.14074104111236166Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGGAAACTCTGGTGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC252410.13906614283488433Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC246650.13589265136176942No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT245040.13500561641876335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA242550.13363374250069804TruSeq Adapter, Index 7 (97% over 35bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG232090.1278707701380623TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT228930.1261297574548951TruSeq Adapter, Index 10 (100% over 38bp)
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA216350.11919876392507123TruSeq Adapter, Index 7 (97% over 35bp)
GAGGAAACTCTGGTGGAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT206170.11359005850904522Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT189690.10451034679429978Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG189300.10429547497580764TruSeq Adapter, Index 7 (97% over 36bp)
AGCTTGACTGGAGAAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC188630.10392633621070574Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT186360.10267567203640525Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)