FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20062914
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC10262185.114999745301206No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT8927834.4499168964189355No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC7813363.8944292937705858TruSeq Adapter, Index 5 (100% over 50bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA3991371.9894268599267284Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1765240.8798522487810095No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG1321650.6587527614383434Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT703230.3505123931648214Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC670230.3340641344522536No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT590160.2941546776305775No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA580560.28936972964146684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG574790.28649377652717845TruSeq Adapter, Index 5 (100% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC501470.24994873626034583No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA493810.2461307465107013Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC457400.22798283439783473No Hit
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA454500.22653738135945756Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG449740.2241648446481902TruSeq Adapter, Index 5 (100% over 37bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA431250.21494883544833018Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG420890.2097850790767483Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG413110.20590727747723986No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC412060.20538392379093084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA406740.20273226511363204TruSeq Adapter, Index 5 (100% over 36bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC364740.18179811766127293Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC353230.17606116439516215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT340240.1695865316473968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG326390.16268324730894027No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT325390.16218481522674125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC307550.1532927868803106Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA276910.13802082788173245No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA273480.13631120583978978Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA272330.1357380089452609TruSeq Adapter, Index 5 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT261460.13032005221175746No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG254150.1266765136908826TruSeq Adapter, Index 5 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT250430.1248223463451022No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG244820.12202614236396568Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC237490.11837263520144681Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC234900.11708169610855133Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG219530.1094207950051523Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAGTGTGACAATGGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAG214150.10673923040292153Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC210080.10471061182837149Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)