FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18335967
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC8504794.638310049314552No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC5343782.9143704283499203TruSeq Adapter, Index 7 (100% over 50bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA3587851.9567279980379544Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT3540081.930675376979027No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1117480.6094469956234105Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT626100.3414600386224517No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC514660.28068331492961346No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA376550.20536140799119024TruSeq Adapter, Index 7 (100% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC310950.16958472929188845No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC297020.16198763883028366No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA254230.13865099124578487TruSeq Adapter, Index 7 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT251430.13712393788666832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA208020.11344915705836514Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG206270.11249474870891729Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA188670.10289612759447048Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC187160.10207260953294692Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT184360.10054555617383037Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)