FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18747076
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC11996696.399232605660744No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT7817074.169754259277553No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3716341.9823571419884358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA2149131.1463814410311242Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1623650.8660817292254004No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1319840.7040244569339773No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC926150.4940237080171862TruSeq Adapter, Index 10 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT914040.48756403398588666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC879180.4689691341732439No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC843710.4500488502847057No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT663810.35408721872146887No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC632330.3372952667391971No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT558130.2977157611138932Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAAC546190.29134676789063Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC521090.2779580132923129No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC519790.27726457181909325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG439000.23416985134108378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAA430300.229529127635691Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC421070.2246056931758318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT407250.21723387689898946No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT390690.20840049936320737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA348000.18562894821571108Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT329150.17557404685402672No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG326560.1741924980727661Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT321690.17159475963078188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC321500.17149341049238825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT314540.16778083152807405Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCCGTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGT312770.16683668429145962No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG304150.16223863390749577No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC283230.15107956035383865Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT281630.15022609392526068Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGA279570.1491272558984665Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAACTC254920.13597853873318697Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGTGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTG244940.13065504188493182Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG239900.12796662263491118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAACT235920.12584362489382342Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG222360.11861049691162505No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA209070.11152139138924917No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT199040.10617122371510096Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA198330.10579249798741948Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAGTAGGACATCGTCAGGCTAGATCGGAAGAGCACACGTCTGAACTCC194600.10380285437579706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT188550.10057568444273657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)