FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17506796
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC9844655.62333050547913No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA7832424.4739311522222565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT4746202.7110614643593265No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2401241.3716044900506066TruSeq Adapter, Index 5 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1368760.7818449475278058No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA940230.5370657200780772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT897600.5127151764377674No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC890310.5085510792494526No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT784950.44836873634673075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG771920.4409259124285221Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC622720.3557018657211748No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT551850.31522044353518486Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC545590.3116446892966594No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA489330.27950859768972003Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG400100.22853981962204847Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA372520.21278593752963132Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC369250.21091809146573706Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC333500.19049745024732112Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG315080.1799758219607974TruSeq Adapter, Index 5 (100% over 37bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC313270.17894193774806083Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA298440.17047094168458923TruSeq Adapter, Index 5 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT285080.16283961953974901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG268730.15350038922027764TruSeq Adapter, Index 5 (100% over 37bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG263630.15058723480869943TruSeq Adapter, Index 5 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT245230.1400770306571231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA236350.13500471474049278Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT231880.1324514205797566No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT223560.12769898044165248TruSeq Adapter, Index 5 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG220120.1257340292307056No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT219900.1256083637462846No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC211670.12090733221544366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA206530.11797132953397069TruSeq Adapter, Index 5 (100% over 40bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG205750.11752578827102345Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACGTCTGAACTCCA202190.11549229225039236Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA199890.11417851673144533Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT183380.10474789333239504No Hit
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA181600.10373114532207948Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG177990.10166908896408TruSeq Adapter, Index 5 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)