FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18570146
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC13802017.432364829011037No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT9870445.315219384920291No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2288341.2322681792593337TruSeq Adapter, Index 10 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1722240.9274240493316531Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1240550.6680345970354783No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC777090.4184619765509652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT692500.372910369148417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC547880.2950326831032993No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG526130.2833203357690349Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC510660.2749897604466868No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC363800.19590583725082183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC362490.19520040391712593Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT343380.18490969322481363No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC321000.1728580916919016No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT317630.17104335097850065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC291610.15703161407562438Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG286750.15441451025748532No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA283140.15247052984936144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG240720.12962741380708584No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC202910.10926677690094627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT197610.10641273364248185Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)