FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17238256
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC13628047.905695332520876No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT7827434.540731962676503No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC5869843.405124045031006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4988862.8940630653124075TruSeq Adapter, Index 1 (100% over 50bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG3441481.9964200554858913Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT2647541.535851422556899Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1554790.901941588522644TruSeq Adapter, Index 1 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT1334530.7741676420166866Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA1182980.6862527160520182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1133230.6573924879639796Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC1108880.6432669290907386Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1092050.6335037604732172Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA1079350.6261364258658184Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1068090.619604442583983TruSeq Adapter, Index 1 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1014460.588493406757621No Hit
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC948000.5499396226625246TruSeq Adapter, Index 1 (100% over 38bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC827670.4801355775201389TruSeq Adapter, Index 1 (100% over 36bp)
GGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA810390.47011136161337896TruSeq Adapter, Index 1 (100% over 37bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG791270.45901975234617703Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC635850.36885981969405723No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAAGATCGGAAGAGCACACGTCTGAAC622480.3611038146782366Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT566760.32878035921963333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG517540.30022758682780903TruSeq Adapter, Index 1 (100% over 39bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC514140.2982552295313401TruSeq Adapter, Index 1 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA497230.28844565250684295Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA470070.27268999833857904TruSeq Adapter, Index 1 (100% over 40bp)
CGGAGGAGAAGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA463570.26891931527180013TruSeq Adapter, Index 1 (100% over 37bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC449170.2605658020161668TruSeq Adapter, Index 1 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG437640.25387719036078826Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA378020.21929132506211765TruSeq Adapter, Index 1 (100% over 40bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC364410.2113960948253698TruSeq Adapter, Index 1 (100% over 36bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA357090.20714972558708955TruSeq Adapter, Index 1 (100% over 37bp)
AGCGGGGCTGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC338950.1966266193053404TruSeq Adapter, Index 1 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT334560.19407995797254665No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT334170.1938537169885399Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG325120.18860376594940928TruSeq Adapter, Index 1 (100% over 39bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT310400.18006461906587304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG298110.17293512754422488Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA297010.17229701194830846Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT284620.16510950991794066No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT278060.16130402054592993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG256580.14884336327294362Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC245860.1426246367381944TruSeq Adapter, Index 1 (100% over 38bp)
AAGCGGGGCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC241320.13999095964232114TruSeq Adapter, Index 1 (100% over 38bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC231070.13404488249855437TruSeq Adapter, Index 1 (100% over 38bp)
AGGGCTGGGTCGGTCGGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCC227660.13206672415121345Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC227460.1319507031337741Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGTAGATCGGAAGAGCACACGTCTGAACT227170.13178247265848703Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCTGGGTCGGTCGGGCTGGGGCTAGATCGGAAGAGCACACGTCTGAAC220230.12775654335334152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT211800.12286625746827289TruSeq Adapter, Index 1 (100% over 41bp)
GAAGCGGGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC197740.11470997994228652TruSeq Adapter, Index 1 (100% over 36bp)
CGAAGCGGGGCTGGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC188570.10939041629269225Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT185600.1076675041837179TruSeq Adapter, Index 1 (100% over 35bp)
AGCGGGGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC179960.10439571149192818TruSeq Adapter, Index 1 (100% over 38bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC178130.10333411918235812TruSeq Adapter, Index 1 (100% over 38bp)
GAAGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA173220.10048580320422204TruSeq Adapter, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)