FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19492650
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA9849375.052863515222404Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC2399201.2308228999135573No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1806830.9269288680605253No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT823440.4224361490100114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC697820.3579913454558513TruSeq Adapter, Index 2 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC644640.3307092673392279No Hit
TCACCGGGTGTAAATCAGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA506910.2600518657032266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT283070.14521883889568632No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT262840.13484056811157027Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATTGCAGAATTCCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC243610.1249753112070447Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTAGTGACGCGCATGAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTC208940.10718912000164163Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC207300.1063477772391132No Hit
TGAATTGCAGAATTCCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC202260.10376218728597703Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)