FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32319516
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT30235949.355319553671535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC27915748.637425139658651No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT17996765.568387843431814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT15956474.93710054321358No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC15177384.696041859042691No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT10350573.202575805900064No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC10154313.1418508866283763No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC9927673.071726074115714No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT6807632.106352706519491Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA5179851.602700362220771Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG3741401.1576287219152663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT3633831.1243454264599755No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT3055200.9453111859719681Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC2945380.9113317167249658No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT2516590.7786595566592025No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT2439900.7549308597319341Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC2379430.7362208023164704No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC2318650.7174148276230374No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1988840.6153681261810975No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG1604570.49647092487399874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTCT1534650.4748369375333467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTGTTGGTTTTCGGAACTGAGGAGATCGGAAGAGCACACGTCTGAACTC1518110.4697192866378321Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1406980.43533448953876663No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT1270110.3929854642625218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1144460.3541080256276115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT1100190.3404104195124704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1079620.33404584400335696No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG1057150.32709338840346497Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT1022120.31625473599295234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG853360.26403860750885005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTAGATCGGAAGAGCACACGTC808040.2500161202909103No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT801970.24813799810615975No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC791650.24494488098150974No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT758230.23460437959528851No Hit
TTTTGTTGGTTTTCGGAACTGAGGCAGATCGGAAGAGCACACGTCTGAACT739120.22869154352435228Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAA709870.21964128423210297Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT693080.21444628069306484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA628680.19452023972141166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC627660.1942046409358358No Hit
TTTTGTTGGTTTTCGGAACTGAGAGATCGGAAGAGCACACGTCTGAACTCC627080.19402518280286127Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC626230.1937621838148814No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG615650.19048861994096694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG596720.18463147777336764No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT571050.17668890833637485Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACGT556550.17220245501201192No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGT548170.1696095944011043No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTAGATCGGAAGAGCACACGTCT515730.15957231537749514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA498000.15408646589880864Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACGT489560.15147504065345532No Hit
TCTATTTTGTTGGTTTTCGGAACTGAGAGATCGGAAGAGCACACGTCTGAA476540.14744651497875155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTCTATTTTGTTGGTTTTCGGAACTGAGGAGATCGGAAGAGCACACGTCT476000.14727943326874077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTG469480.14526207632564794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT466160.14423483321965588No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTCC440160.13619015829321204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC428300.1325205488844573No Hit
TCTATTTTGTTGGTTTTCGGAACTGAGGAGATCGGAAGAGCACACGTCTGA426920.1320935622922076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC425670.1317067990745901No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAA404210.125066848154533Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGA398220.12321347881570999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA395170.12226977656472332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT381980.11818865109242355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGTAGATCGGAAGAGCACACG374010.11572264881689442No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACGTC357400.11058333918119319No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC350500.10844840621994463TruSeq Adapter, Index 8 (100% over 50bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC347200.10742735132543446No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTCT336550.10413212871133341Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT333210.10309869739385949Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT330760.10234064148732921No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)