FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11091778
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC111043710.011352553215543No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT5359244.831723101562256No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2849442.5689659493725894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2145261.9340992940897301No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1541411.3896870276343432No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT1409761.2709955067618555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT819090.7384659159243901No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC761800.6868150444410265TruSeq Adapter, Index 11 (100% over 50bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC723200.6520144921760965No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT685210.6177638968251978No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG600460.5413559485233116Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT598520.5396069052229499Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC560340.5051850118168612No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT447700.4036323121504956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC440880.39748361353788364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC430920.38850398917107787No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC427940.38581731441072836No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC387570.3494209855263962Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA339990.3065243462319567Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG326750.2945875764913434Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC290370.26178850676600274Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA283560.255648823840506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT251190.2264650446483873Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC214130.19305290819920845No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT200960.18117924826840207No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT198920.1793400480968876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT196740.17737462830575945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG185570.16730410579800642Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA182350.1644010545468905Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG179980.16226433670057228No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT176340.15898262659061513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT169910.1531855397754986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG159780.14405264872773327Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT143040.12896038849677663No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTCT137480.12394766646068828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC128320.11568929706310385Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG127160.11464347735773292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC114860.10355418220595472No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT114630.10334682140230358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT114450.10318453903422878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT111620.10063309958060827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)