FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16912312
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT9148245.409219035221204No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC8691285.139025344376334No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC7053684.170736679881497TruSeq Adapter, Index 5 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT4586552.711959192805809No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT3408102.0151591337718937No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1929611.140949859486982No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1848601.0930498443973833No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1595970.9436734610856281No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1482840.8767813649606274No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1063090.6285893968843527Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC827490.48928260074672225No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT816350.4826956834760381No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT787290.4655129351918295No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT778160.46011450119888986No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT712160.4210896771535435Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT686830.40611242271310977No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACG625480.369837074907322No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG589710.34868680284516984No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC568850.33635259330598916No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGTC546190.32295407038375357No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA535990.31692296121310914No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT486870.28787903155996647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT478210.2827585016170468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT443680.26234142321877696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC439680.25997628236754383No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC422710.24994217230618734No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC395270.23371730606672822Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACGT303220.17928950222772616No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT293980.17382602686137769No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG258250.1526994062077379No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT255170.1508782477522884Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACACG219240.12963337005608694No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC178180.10535519921817903No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT177810.10513642368943998Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT174520.10319109533930074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT170790.10098560149552585Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)