FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001839669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279636
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC22077378.95013517572845TruSeq Adapter, Index 10 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC27580.9862821668168619No Hit
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACTAGCTTATCTCGTATGC12080.4319901586347967TruSeq Adapter, Index 10 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGTACTCCAGTCACTAGCTTATCTCGTATGC7750.2771460040910326TruSeq Adapter, Index 10 (98% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT7300.26105365546639203No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6730.24067001387518058No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATTTCGTATGC6710.23995479838075212TruSeq Adapter, Index 10 (98% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC5220.186671244045831No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC4290.1534137235549071No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC3960.14161266789683732No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC3900.13946702141355188TruSeq Adapter, Index 10 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3740.13374529745812413TruSeq Adapter, Index 10 (100% over 49bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC3670.13124204322762448TruSeq Adapter, Index 10 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGGACTCCAGTCACTAGCTTATCTCGTATGC3460.12373228053612555TruSeq Adapter, Index 10 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTTATCTCGTATGC3160.11300404811969847TruSeq Adapter, Index 10 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTTATCTCGTATGC3130.11193122487805575TruSeq Adapter, Index 10 (98% over 50bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2930.10477906993377104TruSeq Adapter, Index 10 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)