FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001839670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980300
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC80835682.46006324594511TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACTGACCAATCTCGTATGC29130.29715393246965216TruSeq Adapter, Index 4 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATTTCGTATGC18930.1931041517902683TruSeq Adapter, Index 4 (98% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG14480.14770988472916455TruSeq Adapter, Index 4 (100% over 49bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC13170.1343466285830868TruSeq Adapter, Index 4 (98% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC12950.13210241762725694No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC11490.11720901764765887TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC10620.10833418341324083TruSeq Adapter, Index 4 (100% over 51bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC10510.10721207793532592TruSeq Adapter, Index 4 (100% over 50bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC10350.10557992451290421TruSeq Adapter, Index 4 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)