FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19365751
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC18106769.349887850979805No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT12003836.198484117656991No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT6253963.2293919301141485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2760591.4255011334184768No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1791200.9249318552118118Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1644430.8491434181922509No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1337090.6904405617938597TruSeq Adapter, Index 12 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1128710.5828382281688946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG874040.45133287110838094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC759970.3924299140270883No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT714370.3688831897095031No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC688380.35546258959954613No Hit
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG519430.2682209432518264Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT515090.26597987343738955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA504470.26049596527395197Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC499140.2577436836815675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC465180.2402075705713659Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT449470.23209531094353117No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC402300.20773787703869578No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG384940.19877359778094844Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC374620.19344460227749494No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG362710.18729456967612565Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT331430.17114234299511544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG316290.16332441742125053No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA281370.14529258379909976Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT275170.1420910554927614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC264460.13656067353132859No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT255940.13216115398777978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA251390.12981164531135406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA233540.12059434204229932No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT232730.1201760778603422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT224390.11586950591278386No Hit
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT219570.11338057584237245Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG213690.11034428770668382No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC207800.10730283581566241No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC196520.10147811980025974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)