FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29910418
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT346896511.597848615823423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC17142635.731324115898347No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC16537855.5291270085225825No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT15387975.144685707835979No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT13534634.525055450579126No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC12660054.232655658640411No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA11991764.009225146903664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC8058062.6940646566691244No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT7404922.4756992697327065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT6473942.164443171606629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG4463321.4922292292939536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT4219911.4108495575020048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC4091991.3680818502770506No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT2621660.876503965942569No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT2172870.7264592557683414No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT2078180.6948013899371115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1804380.6032613786942062No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAA1716760.5739672377697964Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA1593800.5328578156279862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC1553170.5192739198763454No Hit
CTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACACGTCTGA1370350.4581514039690118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1309070.4376635592321043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1302280.43539344719288103No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT1222410.4086903767108839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC1153340.3855980882647645No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC1096660.3666481692098051No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT1079370.3608675746357005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAAGATCGGAAGAGCACACGTCTGAA987120.33002547807924315Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAA912730.30515454514878393Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG835390.2792973337918581Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG818860.2737708312869449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT786710.2630220680968083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA767360.256552750282527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC734140.24544625220550242No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC712930.23835507748504214No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTCT676230.22608510519645697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG660820.22093305416193113Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG657780.2199166858851655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTG652410.21812132481732618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT619010.2069546470397037No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA598390.20006072800453673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC570790.1908331739128487No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT518900.17348470355713516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAGATCGGAAGAGCACACGTCTGAAC502980.16816214337091512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACGT502430.16797826095242133No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGA481920.16112111840095314Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT480320.16058618772897124No Hit
GCATTGGTGGTTCAGTGGTAGAATTCAGATCGGAAGAGCACACGTCTGAAC475360.1589279026458273Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC456690.1526859303671383No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTTAGATCGGAAGAGCACACGTCTGA424280.1418502409428046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT417430.13956006900338203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACACGTCTGAA397910.13303391480520266Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTCGGGCTGGGGCGCGAAGCGGGGCAGATCGGAAGAGCACACGTCTGAA367000.12269972288585201Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGGTCGGGCTGGGGCGCGAAGCGGGGCAGATCGGAAGAGCACACGTCTG366250.12244897413336048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT341420.11414751876754113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC324450.10847391032783293No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC322920.10796238287275023No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACGT314100.10501357754344992No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC308860.10326167959270913Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)