FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16408798
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC11391336.942208685852553No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT8551995.211832091540161No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT5474493.3363138482172796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA4402422.6829631274636934TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA4260302.5963510550864237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2447271.4914377031151216No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1659361.0112623727831862No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1331440.8114183622712644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG1098010.6691593131928372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC790720.4818878262746607No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT781900.47651266107365087No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG781610.47633592661692825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC758110.462014341330791TruSeq Adapter, Index 7 (100% over 50bp)
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA695190.42366905851360964TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAA620420.37810204013724835Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC611560.372702497769794No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC508510.3099008227171789Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT495270.30183198062405303No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC473350.28847329341247296No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAAC471580.28739460379730436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA451060.2748891174112814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT443300.2701599471210505Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT415350.2531264020679638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT375080.22858468974997437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT370430.22575084415080252Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT366020.2230632615502976No Hit
GGGGCCGTAGCTCAGCTGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT361880.220540224823293Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAACTCGGGAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC352260.21467751629339332Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA335570.20450614359443028Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT331220.20185512674359204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC330870.2016418265372028No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA315860.19249429482890826Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG282130.1719382492245928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG267640.16310762068007664No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG258450.15750696668945524Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC248390.15137610932866624No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT235400.1434596245258184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT225520.1374384644140296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT224370.13673762087875052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT213770.13027767177096092Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG204340.12453075478167262No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT199810.12177004068183422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT194260.11838770883766137No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC191900.1169494560174365No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG190300.11597436935965692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG190190.11590733215193459Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTAGATCGGAAGAGCACACGTCTGA189550.11551729748882275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA189180.11529180869921124Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAACT184820.11263469755676192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAAGATCGGAAGAGCACACGTC179480.10938034583642263No Hit
GGGGCTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA172460.1051021531254148TruSeq Adapter, Index 7 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)