FastQCFastQC Report
Wed 8 May 2024
EGAF00001839699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25807341
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGTGACGATGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT6901872.674382455751641Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGTGACGATGGCAAGAAGGAGATCGGAAGAGCACACGTCTGAACTCC4049361.5690729238630203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAAC3452611.3378402680074635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAA3179041.2318355463276902Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT2456540.9518764447681766No Hit
GTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTC2393900.927604281277951Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACT2345400.9088111789587312Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGTGACGATGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1973690.7647785178643549Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGTGACGATGGCAAGAAGAGATCGGAAGAGCACACGTCTGAACTCCA1953190.7568350416263341Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGTGACGATGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1560910.6048317802287342Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGTGACGATGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1544340.5984111265085388Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACTCGTTAGCTCAGTTGGTAGAGCATCTAGATCGGAAGAGCACACGTCT1429990.554102028566213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCATGCCGAACAGATC1416170.5487469631218497No Hit
CGCGGAGTGGAGCAGTTAGGTAGCTCGTCGAGATCGGAAGAGCACACGTC1343650.5206464315715439No Hit
TGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCC1234740.47844526098213686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCCAG1175670.4555564248172642Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC1096150.4247434867466586Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG988520.38303829906382064No Hit
CTTTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGA932960.3615095410255555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGTGACGATGGCAAGAAGGAAGATCGGAAGAGCACACGTCTGAACTC894020.3464208110397735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTAGATCGGAAGAGCACACGTCT877160.3398877861923086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGACGATGGCAAGAAGGATAAGATCGGAAGAGCACACGTCTGAAC862860.3343467271579819Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGGTTAAGAGGATGGGTTAGCAGATCGGAAGAGCACACGTCTGAACT834180.3232336101576679Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA787720.3052309805957925Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACCTAGATCGGAAGAGCACACGT786060.3045877527638357No Hit
TTAAGTGAATAAGGGCGCATGGTGGATGAGATCGGAAGAGCACACGTCTG764100.2960785460230095Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCATGCCGAACACAGA719500.2787966416222423No Hit
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGAGATCGGAAGAGCACACGTC673230.2608676345230607No Hit
TGTGGTGACGATGGCAAGAAGGATACAAGATCGGAAGAGCACACGTCTGA638200.24729397732218905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCAGATCGGAAGAGCACA626450.242741009234543No Hit
TTAAGTGAATAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC624690.24205903273801047Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAAC607630.23544851056139415Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGTGACGATGGCAAGAAAGATCGGAAGAGCACACGTCTGAACTCCAG576750.22348292294041452Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAAGTAGCTCAGCTTGGTAGAGTAGATCGGAAGAGCACACGTCTGAA574660.22267307585078214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG571810.22156873891037437No Hit
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCC570440.22103788220568713Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAA566080.21934844043018614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTACCGAAGCTGTGGATAGAACAGATCGGAAGAGCACACGTCTGAACTC558190.21629117079516252Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTCGTTAGCTCAGTTGGTAGAGCATCTGAGATCGGAAGAGCACACGTC557310.21595018254689627No Hit
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCATGCCAGATCGGAA526840.20414346445067705No Hit
ACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTTAGATCGGAAGAG521740.20216728255731575No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGTAAGATCGGAAGAGCACACGTCTGA504270.195397890855939Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTTTCCCTTTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCAC498950.19333646190051118No Hit
TGTGGCGATGATGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT494310.19153852386419817Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGTGTAGCTCAGCTGGCTAGAGCGTCCAGATCGGAAGAGCACACGTC488720.1893724735144159No Hit
TGTGGTGACGATGGCAAGAAGGATAGATCGGAAGAGCACACGTCTGAACT441210.1709629829744955Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAACTC427100.16549554640286265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAAGTGAATAAGGGCGCACGGTGGATGCCTTGGCAGATCGGAAGAGCAC414570.16064033873152603No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACT405930.1572924541121846Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGTGACGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC404410.1567034744106338TruSeq Adapter, Index 1 (100% over 36bp)
TGTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAACT402880.1561106198426254Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCAGATCGGAAGAGCA392150.1519528881336516No Hit
GGTTCCGTGGTGTAGGGGTTAACATGCCTGAGATCGGAAGAGCACACGTC386800.14987983457885104No Hit
AGGGGTATAGTTTAGCGGTAGAACTACGAGATCGGAAGAGCACACGTCTG381360.14777190722593234Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAAGGTCATTGGAAACTGGGGAACTTGAATGCAGATCGGAAGAGCAC372440.1443155263457789No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGCCAAGGTAGATCGGAAG370640.1436180503834161No Hit
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCAGATCGGAAGAGCAC363270.14076227380418618No Hit
GAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCCA361300.13999892511204468Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGCTACACACGTGCTACAATGGACGGTAGATCGGAAGAGCACACGTCT358090.13875509297916433Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTCGGGACCTAAGCTGAGGCCAGATCGGAAGAGCACACGTCTGAACTC357060.13835598173403452Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTGAGAGTAGGACGTTGCCATGCAGATCGGAAGAGCACACGTCTGAAC355650.13780962556351697Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGTGACGATGGCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA349250.13532971103067148Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCTACACACGTGCTACAATGGACGGTAAGATCGGAAGAGCACACGTC342950.13288854516240164No Hit
GCGGAAGTAGTTCAGTGGTAGAACAAGATCGGAAGAGCACACGTCTGAAC339660.13161371409786077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTAGATCGGAAGAGCACAC332640.12889355784464582No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCAAGATCGGAAGAGCACACGTCTG326810.12663451069988188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC326580.12654538877135774No Hit
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGAAGATCGGAAGAGCACACGT322650.12502256625353228No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCAGATCGGAAGAGCACACGTC309340.11986511899850512No Hit
TTTGTGAGAGTAGGACGTTGCCATGCAGATCGGAAGAGCACACGTCTGAA309030.1197449981383204Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCCAGT300030.1162576183265064Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGATTGTAGGCTGCAACTCGCCTACAAGATCGGAAGAGCACACGTCTG299730.11614137233277927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTGACGATGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA297080.11511453272152292Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT295740.1145953006162084No Hit
GACTCGTTAGCTCAGTTGGTAGAGCATCTGACTAGATCGGAAGAGCACAC289840.11230912940624142No Hit
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAG285540.11064293682948585Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGAAGGTCATTGGAAACTGGGGAACTAGATCGGAAGAGCACACGTCTG282060.1092944833022511Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGAGAGTAGGACGTTGCCATGCAGATCGGAAGAGCACACGTCTGAACTC277900.10768253885590151Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTTCCGTGGTGTAGGGGTTAACATGCCTAGATCGGAAGAGCACACGTCT274290.10628371206471833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACAGATCGGAAGAGCACACGTCT272890.10574123076065836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGACGATGGCAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT272710.1056714831644221Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGCGATGATGGCAAGAAGGAGATCGGAAGAGCACACGTCTGAACTCC271560.10522587352180142Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGTGTGGCGGAATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAG270780.10492363393811086Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTAAGTGAATAAGGGCGCATGGTGAGATCGGAAGAGCACACGTCTGAACT269680.10449739862777804Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATTAGATCGGAAGAGCACAC267340.1035906798767064No Hit
GTGTGTCGGCAGTTCGATTCTGTCTTGCAGATCGGAAGAGCACACGTCTG261270.10123863593696072Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTGACGATGGCAAGAAGAAGATCGGAAGAGCACACGTCTGAACTCC259350.10049466157710707Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)