FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25807341
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGTGACGATGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC6889172.6694613753505254Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTGGTGACGATGGCAAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCA4038911.5650236884148585Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACT3447771.335964832641999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAAC3173851.2298244906362108Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC2450190.9494159045676189No Hit
GTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCC2388710.9255932255864716Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTC2342000.907493724363157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGTGACGATGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1970650.7636005584612533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGTGACGATGGCAAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAG1949330.7553393431737117Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGGTGACGATGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1559630.604335797322165TruSeq Adapter, Index 1 (100% over 35bp)
TGTGGTGACGATGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1542510.5977020259468032Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACTCGTTAGCTCAGTTGGTAGAGCATCTAGATCGGAAGAGCACACGTCTG1425130.5522188434678335Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCATGCCGAACAGATCG1412840.5474566325914785No Hit
CGCGGAGTGGAGCAGTTAGGTAGCTCGTCGAGATCGGAAGAGCACACGTCT1341690.51988695774586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCCA1231490.47718592938342624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1173410.4546807049978532Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1093660.4237786449987234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT987130.38249969262621825No Hit
CTTTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAA931000.3607500671998715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGTGACGATGGCAAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCC892160.3457000858786653Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTAGATCGGAAGAGCACACGTCTG874370.33880669845064626Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTGACGATGGCAAGAAGGATAAGATCGGAAGAGCACACGTCTGAACT861560.3338429945184977Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGGTTAAGAGGATGGGTTAGCAGATCGGAAGAGCACACGTCTGAACTC833110.32281899944670783Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG786130.3046148768290387Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACCTAGATCGGAAGAGCACACGTC784570.3040103976616576No Hit
TTAAGTGAATAAGGGCGCATGGTGGATGAGATCGGAAGAGCACACGTCTGA763010.29565618557913426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCATGCCGAACACAGAA704210.2728719708086161No Hit
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGAGATCGGAAGAGCACACGTCT672040.26040652541460974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGACGATGGCAAGAAGGATACAAGATCGGAAGAGCACACGTCTGAA636920.24679799441561998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCAGATCGGAAGAGCACAC625340.2423108990577526No Hit
TTAAGTGAATAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA623820.2417219193562018Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAACT606800.23512689664541572Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGTGACGATGGCAAGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT575580.22302956356487869Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGAAGTAGCTCAGCTTGGTAGAGTAGATCGGAAGAGCACACGTCTGAAC573870.22236696140063403Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT571070.2212819987925141No Hit
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA569030.22049152603516958Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAAC565160.2189919527160896Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTACCGAAGCTGTGGATAGAACAGATCGGAAGAGCACACGTCTGAACTCC557200.21590755901586298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTCGTTAGCTCAGTTGGTAGAGCATCTGAGATCGGAAGAGCACACGTCT556540.21565181782966328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCATGCCAGATCGGAAG525410.20358935854724436No Hit
ACTGAACAAAGTAAGACAAACCAAATGTGTAGGGCGTTAGATCGGAAGAGC520920.20184954350779494No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGTAAGATCGGAAGAGCACACGTCTGAA503240.1949987796108092Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGTTTCCCTTTGTGAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACA497890.19292572605600863No Hit
TGTGGCGATGATGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC493500.19122465968113492Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGTGTAGCTCAGCTGGCTAGAGCGTCCAGATCGGAAGAGCACACGTCT487940.18907023393072536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGACGATGGCAAGAAGGATAGATCGGAAGAGCACACGTCTGAACTC440740.17078086425098968Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAACTCC426140.1651235592229358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAAGTGAATAAGGGCGCACGGTGGATGCCTTGGCAGATCGGAAGAGCACA413730.16031484994909007No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC405330.1570599621247303Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGTGACGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA403660.15641285942631594TruSeq Adapter, Index 1 (100% over 37bp)
TGTGAGAGTAGGACGTCGCCATGTAGATCGGAAGAGCACACGTCTGAACTC402290.1558820027216287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCCAGATCGGAAGAGCAC391470.15168939721453675No Hit
GGTTCCGTGGTGTAGGGGTTAACATGCCTGAGATCGGAAGAGCACACGTCT386320.14969384098888763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGTATAGTTTAGCGGTAGAACTACGAGATCGGAAGAGCACACGTCTGA380630.14748904197452964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGAAGGTCATTGGAAACTGGGGAACTTGAATGCAGATCGGAAGAGCACA371750.1440481605602065No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGCCAAGGTAGATCGGAAGA369830.14330418620035285No Hit
TGTGGTGACGATGGCAAGAAGGATACACCTGTTCCAGATCGGAAGAGCACA362540.14047940855278349No Hit
GAGAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCCAG360640.13974318392584498Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGCTACACACGTGCTACAATGGACGGTAGATCGGAAGAGCACACGTCTG356920.13830173360362852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTCGGGACCTAAGCTGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC356250.13804211755097126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTGAGAGTAGGACGTTGCCATGCAGATCGGAAGAGCACACGTCTGAACT355060.13758100844252028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGTGACGATGGCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC348820.1351630917729959Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGCTACACACGTGCTACAATGGACGGTAAGATCGGAAGAGCACACGTCT342380.1326676777743201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAAGTAGTTCAGTGGTAGAACAAGATCGGAAGAGCACACGTCTGAACT339290.13147034403893063Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGTGACGATGGCAAGAAGGATACACCTGTTAGATCGGAAGAGCACACG332010.12864944125781885No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCAAGATCGGAAGAGCACACGTCTGA326290.12643301764408815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT326040.1263361459826489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGAAGATCGGAAGAGCACACGTC322020.1247784496667053No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCAGATCGGAAGAGCACACGTCT308730.11962875214459327Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGTGAGAGTAGGACGTTGCCATGCAGATCGGAAGAGCACACGTCTGAAC308310.11946600775337528Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTAGGACGTCGCCATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC299440.1160290012055097Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGGATTGTAGGCTGCAACTCGCCTACAAGATCGGAAGAGCACACGTCTGA299100.11589725574595229Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGTGACGATGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT296850.11502541079299877TruSeq Adapter, Index 1 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC295170.11437443322812683No Hit
GACTCGTTAGCTCAGTTGGTAGAGCATCTGACTAGATCGGAAGAGCACACG289390.11213476041565074No Hit
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT285060.11045694323952243Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGGAAGGTCATTGGAAACTGGGGAACTAGATCGGAAGAGCACACGTCTGA281640.1091317389110331Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGAGAGTAGGACGTTGCCATGCAGATCGGAAGAGCACACGTCTGAACTCC277370.10747717093365022Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTTCCGTGGTGTAGGGGTTAACATGCCTAGATCGGAAGAGCACACGTCTG273280.10589235055250364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTGACGATGGCAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC272190.1054699901086284Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACAGATCGGAAGAGCACACGTCTG272000.10539636764593453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGCGATGATGGCAAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCA270810.10493525853748358Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGTGTGGCGGAATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT270320.1047453900810626Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTAAGTGAATAAGGGCGCATGGTGAGATCGGAAGAGCACACGTCTGAACTC269250.10433077937010249Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATTAGATCGGAAGAGCACACG266800.10338143708799757No Hit
GTGTGTCGGCAGTTCGATTCTGTCTTGCAGATCGGAAGAGCACACGTCTGA260820.10106426694637004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGTGACGATGGCAAGAAGAAGATCGGAAGAGCACACGTCTGAACTCCA258820.10028929365485581Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)