FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19096634
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT230570412.073876474775606No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC17999219.425331186637393No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT11779796.168516399277485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC4893232.5623520878077257No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT3822182.0014940852927277No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT2646621.3859091607452916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2278311.1930427110872TruSeq Adapter, Index 11 (100% over 50bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1761020.9221625130376379Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1751550.917203523929924No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC1615060.8457301951747098Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1426980.747241634311052No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG1354110.7090830771538063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC1270180.665132923425144TruSeq Adapter, Index 6 (97% over 36bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA1169290.6123016234169854Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG1166460.6108196868620931Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT1022000.5351728477385073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT1018980.5335914172099648TruSeq Adapter, Index 11 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT917990.48070775195251686No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC899230.47088403118580996No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT867680.4543627950349784No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT825200.4321180371368064No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG732560.3836068701950302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT732380.3835126127463091Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG570620.2988065854956428Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT550560.2883021164881727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC533630.2794366797834634No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT513910.2691102526235775No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA510820.26749216642053253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT501160.26243368333916856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT471270.24678171032654236Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT458630.24016274281635186Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG409520.21444616889028717Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTAGATCGGAAGA379560.1987575402031583No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC377520.19768928911765288No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT359460.1882321250959724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG343810.18003696358216845Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA341820.17899489512130776Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA323300.16929685095289568Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGAAACTCTGGTGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC313350.1640865086485922Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAAGCTGGGTTGAGAGGGCGAAAGATCGGAAGAGCACACGTCTGAACTCC310410.16254697031948143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG304390.15939458231225462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCTGGGTTGAGAGGGCGATAGATCGGAAGAGCACACGTCTGAACTCC284490.14897389770364766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT281170.14723537142723686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA267000.13981521560291724TruSeq Adapter, Index 11 (100% over 38bp)
AAAAGCTGGGTTGAGAGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA266700.1396581198550488Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC254680.13336381689045307No Hit
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA247100.1293945309943103Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA234380.12273367128468819Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC226310.11850779566702697Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG223270.11691589208862672No Hit
AGGAAACTCTGGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG222170.11633987434644241TruSeq Adapter, Index 2 (97% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG213880.11199879518034433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT212640.11134946608915477No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCCA212000.11101432849370209Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT211870.11094625366962577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGAAACTCTGGTGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT205330.10752156636609364Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTTCAGATCGGAAGAGCACACGTCTGAACTCC205140.10742207239244361Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGACATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGTCTGAACTC204670.10717595572078305Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGGGCAGAGAGCGAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG197960.1036622474934588Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)