FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16873245
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC13350557.912259912067893No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6990304.142830854408858Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT6285353.725039255934469No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2895311.7159177147015883Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA2217781.3143766951762983Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT2079561.2324600276947322TruSeq Adapter, Index 12 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1672050.9909475029847549No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC1394230.8262963051861096No Hit
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1288800.7638127698613989Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC1230520.7292728814167043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1169390.6930439284204076No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG984550.5834977208000003TruSeq Adapter, Index 12 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT945800.5605323694404959No Hit
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC929830.5510676814092369Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT742310.4399331604560948No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC721120.42737481735137495No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC715150.4238366716064397No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC701390.41568174942045827Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG667230.3954366809703765TruSeq Adapter, Index 12 (100% over 37bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG661220.39187482905629595Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC612520.36301256812189947Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC606780.3596107328495497No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT600530.3559066439205974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC590150.3497548930273933TruSeq Adapter, Index 12 (100% over 50bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG526470.312014671748084Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC519430.30784238597851216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA484350.2870520756380886Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT455310.2698413968386046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA452450.26814640574471593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC439700.26059006432965326No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA426690.25287963281514614Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG424490.25157579351215487Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT416630.2469175312751045Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT414860.24586853329042518Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGAGATCGGAAGAGC412160.2442683668731178No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA399280.23663498040833283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT392090.2323737965044661Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG384830.22807112680459507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG379130.2246929977013906Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACA379070.22465743844767264No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG372120.22053849155867764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG363410.2153764732272897No Hit
ACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT349720.20726303683731256TruSeq Adapter, Index 1 (97% over 35bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA347000.20565101733543253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT315030.18670386164605562Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT314810.1865734777157565Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACTC310840.184220640428086Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG297470.1762968533912712No Hit
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT295990.1754197251328953TruSeq Adapter, Index 12 (100% over 38bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC295730.17526563503345088Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA275680.16338291774937186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT244930.1451588002189265No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT242010.14342824987131994No Hit
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCCA210940.1250144829877122Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACAC207300.12285722159549037No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT190450.11287099784303495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT189290.11218351893782139Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG186550.1105596463513687Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG181870.10778602456136921No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT178230.10562876316914736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT176210.10443160162730998Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA173210.10265363894141287Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT170610.1011127379469687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)