FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17093508
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT13928008.14812266738928No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC10981296.424245976893683No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT7713554.512561143095964TruSeq Adapter, Index 8 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT5511223.224159721924839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2159551.2633743758156604No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1970541.15280023269653No Hit
CGGGCGGTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG1188910.6955330643657229TruSeq Adapter, Index 8 (100% over 38bp)
CGGGCGGTGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1187070.6944566323074234TruSeq Adapter, Index 8 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1054270.6167663185344986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA878650.5140255587091895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT855260.5003420011854793TruSeq Adapter, Index 8 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC711570.4162808476762055TruSeq Adapter, Index 8 (100% over 50bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG684710.40056727969472383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT671530.3928567500597303No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT629130.36805201132500126No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC523340.30616301814700647No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT489610.28643038046959113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGCGGTGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT488850.2859857672281196TruSeq Adapter, Index 8 (100% over 36bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA469970.2749406382820893Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC444230.25988228981435524No Hit
ACGGGCGGTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT408460.2389562165940426TruSeq Adapter, Index 8 (100% over 37bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT405990.23751122355926005Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC374720.2192177287423974No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT365130.21360741165593394No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC327500.19159320602886196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG293590.17175526521530862No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG279570.16355332094500438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT273850.16020702128550793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC261570.15302300733120433No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT246860.14441740103903775TruSeq Adapter, Index 8 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT240440.14066158918344907Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC239310.14000051949547163Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT226570.13254739752659314TruSeq Adapter, Index 8 (100% over 37bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG222810.1303477320161549No Hit
CGGGCGGTGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG209490.12255529994194286TruSeq Adapter, Index 8 (100% over 38bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC198670.11622541142520307No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT182280.10663697586241513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC175550.10269980860569988Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)