FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14447554
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC5561723.8495928099663095No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2738101.8951996995477574No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA799550.5534154778033707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA767750.5314048315721817Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT710830.49200715913572635Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC407470.2820338999944212No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT376710.2607430987972082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT374080.2589227214516727No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG357620.2475297894716296Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC268440.18580307780818817Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC251730.17423710615651616No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC248370.17191145296982452TruSeq Adapter, Index 1 (100% over 50bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC233080.16132834665300438TruSeq Adapter, Index 1 (100% over 36bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT195870.13557312192776716No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC171920.11899592138572383Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC168600.11669795454649279No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC162730.11263498305664751No Hit
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA162110.11220584467100797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA148410.10272327066574728Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG147010.10175424850462576Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)