FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524364
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT7930445.863817329968344No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC7303415.400187395133701No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1438111.0633475999315014Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1268380.9378481679434243No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1046530.773810879387748No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA907870.67128480126681Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT807340.5969522855196739No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT717470.5305018409738158No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT591190.43712961289713886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT568680.4204855769927517No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC543070.4015493815457792TruSeq Adapter, Index 12 (100% over 50bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC532370.3936377340923388No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT449410.33229658710753424Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC440170.32546447285802127No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC390030.28839064077246074No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC303670.22453551235385263No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC281300.20799499333203397No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC263100.1945377985981448Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT250760.18541352480604634No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT220850.1632978822516164TruSeq Adapter, Index 12 (100% over 36bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC208030.15381869343356921Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA199000.14714185450790884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT197970.1463802660147272Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG197880.14631371944736182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC188240.1391858426762249No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG182880.13522262488646417No Hit
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC162850.12041231661614549Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC162630.12024964722925234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG155800.1151995021725236Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT152320.11262636823439534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG151930.11233799977581202No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT147150.10880363764240596No Hit
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT144360.10674069405407899Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG143370.10600868181305975TruSeq Adapter, Index 12 (100% over 37bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA135950.10052228703693572Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)