FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22932263
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAGTGGTAAAACCACAAGATCGGAAGAGCACACGTCTGA8259053.601498029217614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGAACAAAGAATAGACGAACCAGATCGGAAGAGCACACGTCTGAACTCC7132073.1100593953592806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT5616132.4490081942632527No Hit
ACTGAACAAAGAATAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC5251222.2898830350933963Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC4522971.9723173417294226No Hit
ACTGAACAAAGAATAGACGAACCAAATGTGTAGATCGGAAGAGCACACGTC3708711.6172455374334402No Hit
CGGGAAGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT3546411.5464718854829111TruSeq Adapter, Index 2 (100% over 37bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC3424201.4931801540912033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG3160001.3779712887472118Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGAACAAAGAATAGACGAACCAAATGTGTAAGATCGGAAGAGCACACGT2889221.2598931034412084No Hit
ACTGAACAAAGAATAGACGAACCAAATGTGAGATCGGAAGAGCACACGTCT2346431.0232003705870634Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC2286090.996888096041808Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA2211500.9643618686912844Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGAACAAAGAATAGACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2030520.8854424877300596Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGAACAAAGAATAGACGAACAGATCGGAAGAGCACACGTCTGAACTCCA1822340.794662088080884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGTGGCGATAGCGAGAAGGATAGATCGGAAGAGCACACGTCTGAACTC1696130.7396260892350659Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGAACAAAGAATAGACGAACCAAATGTGTAGGAGATCGGAAGAGCACAC1643010.7164622174444799No Hit
ACTGAACAAAGAATAGACGAACCAAATGTGTAGGGAGATCGGAAGAGCACA1155780.5039973595279279No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1100710.47998315735346314No Hit
ACTGAACAAAGAATAGACGAACTAGATCGGAAGAGCACACGTCTGAACTCC1058140.46141979097309327Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC936700.4084638310663017No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT916420.3996203950739619Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT851640.3713719836546441No Hit
TGTGGTGGCGATAGCGAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCC799270.34853516201170376Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT748320.32631755531497264Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC731490.31897855000180314TruSeq Adapter, Index 2 (100% over 50bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATTTAGATCGGAAGAGCACAC705830.30778907428368496No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA676060.2948073637564684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGAACAAAGAATAGACGAACCAAATGTAGATCGGAAGAGCACACGTCTG653990.28518336807841427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT647470.28234021212821425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT622000.2712335891141664No Hit
CACATTTCGTCTTTACTTTGTTCAGTTTAGATCGGAAGAGCACACGTCTGA614200.2678322675786511Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT610800.2663496402426573Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTCC586450.2557314121157602Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT579020.25249143531975016No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT544960.23763899794800014Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGGCATAGCTCAGTTGGTAGTAGATCGGAAGAGCACACGTCTGAACTCC524940.22890893934017761Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC520880.22713850787425557No Hit
TTTCGTCTTTACTTTGTTCAGTTTAGATCGGAAGAGCACACGTCTGAACTC518090.2259218813250136Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT503790.2196861251765689TruSeq Adapter, Index 2 (100% over 37bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC501900.21886195880450177Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTTTGGTAGTTCAGCTGGTTAGAATGAGATCGGAAGAGCACACGTCTGAA471720.20570146086323882Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACTC463880.20228269665318246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGAACAAAGAATAGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTC461900.20141928426339784Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGGGTGTGGCGGAATTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT439070.19146387776906273Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA436200.19021236587073853TruSeq Adapter, Index 2 (100% over 36bp)
GAGAAGAACAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC434040.18927046144551893Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGAACAAAGAATAGACGAACCAAGATCGGAAGAGCACACGTCTGAACTC431250.18805383489627692Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGGTCCTGTTCTGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC426000.185764483862757Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGAACAAAGAATAGACGAACCAAATGTGCAGATCGGAAGAGCACACGTC400550.17466658218597964No Hit
ACTGAACAAAGAATAGACGAACCAAATGAGATCGGAAGAGCACACGTCTGA386960.16874043351063958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGGTGGAGCAGTCAGGTAGCTCGTCGGGCTAGATCGGAAGAGCACAC376650.16424458414767004No Hit
GGCGATCGTGGTGAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC362040.1578736472715318Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTTCGTCTTTACTTTGTTCAGTTTAGATCGGAAGAGCACACGTCTGAACT343380.14973663959810682Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGTGGCGATAGCGAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCA341070.14872932514335807Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGAACAAAGAATAGACGAACAAGATCGGAAGAGCACACGTCTGAACTCC337800.14730338649962282Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC321510.14019985729275825No Hit
ACTGAACAAAGAATAGACGAAAGATCGGAAGAGCACACGTCTGAACTCCAG312800.13640171491143285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGGCTTAGCTCAGTTGGTAGAGCATCTGATTAGATCGGAAGAGCACACG296540.1293112677104741No Hit
ACTGAACAAAGAATAGACGAACCAAATGTGTAGGGCAGATCGGAAGAGCAC295900.12903218491781646No Hit
CGGGAAGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT293640.12804667380624407TruSeq Adapter, Index 2 (100% over 37bp)
AAATGGATTTTTGGAAATAGGAAGATCGGAAGAGCACACGTCTGAACTCCA290050.12648119376617997Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTTCGTCTTTACTTTGTTCAGTTTAGATCGGAAGAGCACACGTCTGAAC277920.12119170271159023Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGAACAAAGAATAGACGAACCAAAAGATCGGAAGAGCACACGTCTGAAC276720.1206684224753571Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTGAGAGTAGGACGTCGCCACGCAGATCGGAAGAGCACACGTCTGAACT272250.11871920359538873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGATCGTGGTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC245570.1070849396764724Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCGATCGTGGTGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC239710.10452958785620067Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGGCATAGTTTAAAGGTAGAACAACGAGATCGGAAGAGCACACGTCTGA233250.10171259591781238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)