FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17873515
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC11426536.392995446055238No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT8842154.94706832987244No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2022911.1317919278888344TruSeq Adapter, Index 4 (100% over 50bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1822301.0195532328140269Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1735230.9708386962497304No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1721190.9629834982095017No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1675690.9375268378939453No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1431690.8010120001577753TruSeq Adapter, Index 2 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1236780.691962381210411Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1181020.6607653838654568No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC918700.5140007435582761No Hit
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC676620.37856012093871855TruSeq Adapter, Index 4 (100% over 37bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT646210.3615461200552885Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC536310.3000584943700218No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC528160.29549867499481774Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT510320.2855174262029601Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC476020.2663270207343099TruSeq Adapter, Index 4 (100% over 38bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC465900.26066501189049834Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC465840.2606314426681042No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC439670.24598966683386006No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC437160.24458535436370515TruSeq Adapter, Index 4 (100% over 37bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC377290.21108886528475235TruSeq Adapter, Index 4 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC369210.20656821000234146No Hit
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT363070.20313295957734112TruSeq Adapter, Index 4 (100% over 41bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT350270.1959715254665912No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT341280.1909417369778692No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC334900.1873722096632923Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA319290.17863861697041686TruSeq Adapter, Index 4 (100% over 39bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT312010.17456555131992785Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA305950.17117505985811968TruSeq Adapter, Index 4 (100% over 40bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT292670.16374507196821667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC282380.1579879503276216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG279810.15655006863507262Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT269250.15064188549370397Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG255110.14273073874948491No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC253730.1419586466344197Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA250390.1400899599211459TruSeq Adapter, Index 2 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT241510.13512171500681316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT227140.1270818862434166No Hit
GGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC223820.12522438927094082TruSeq Adapter, Index 4 (100% over 37bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG216790.12129119538042742Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT205270.1148459046807525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA191710.10725926041967682Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC186880.10455693801694854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)