FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001839740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4416026
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1712563.878056877382515No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC876251.9842500927304323No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC760731.7226574300060735TruSeq Adapter, Index 7 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT687521.5568748915880477Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC518831.174879858044314Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA468571.0610671223403123TruSeq Adapter, Index 7 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT270030.6114773780770313No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC238640.5403953690490046No Hit
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA237590.5380176656568598TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT225050.5096210937163866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA207100.4689736881078146Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT205520.46539581062249186TruSeq Adapter, Index 7 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC193190.43747477936044765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC181550.41111623889895577Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACAC175390.39716704566503913No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG172640.39093972725704057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT165030.373707038862543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG157740.3571989838827942TruSeq Adapter, Index 7 (100% over 36bp)
ACGGCCCTGGCGGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA148160.33550527102874844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA144160.326447353344387TruSeq Adapter, Index 7 (100% over 37bp)
ACGGCCCTGGCGGAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC138250.31306427996574293Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC130660.2958768811596671Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC113550.25713163826481095No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG112170.25400665666370625TruSeq Adapter, Index 7 (100% over 36bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA102530.23217707504439514TruSeq Adapter, Index 7 (100% over 37bp)
GCTGTCGGTTGCTGTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT101130.2290068038548686Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC91690.20763011811977555Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG84490.19132586628792492TruSeq Adapter, Index 7 (100% over 36bp)
AGCCGGCGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA83330.1886990701594601TruSeq Adapter, Index 7 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT80910.18321902996042144No Hit
AAAAGCTGGGTTGAGAGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG77750.17606327498977586Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC75720.17146638176496243Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT62720.1420281492907877TruSeq Adapter, Index 7 (100% over 38bp)
AGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC62340.14116764711077334TruSeq Adapter, Index 7 (100% over 39bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG60480.13695571538754528TruSeq Adapter, Index 7 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT57770.1308189761563904No Hit
CGGAGAGGGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA57450.13009434274164147TruSeq Adapter, Index 7 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC55120.12481810569050092No Hit
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT54330.12302916694783954Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA52250.11831904975197158TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT50610.11460530350138338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA49840.11286165434714378TruSeq Adapter, Index 7 (100% over 37bp)
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC49150.11129916354659143TruSeq Adapter, Index 7 (100% over 39bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACA48740.1103707269839444No Hit
TGAGGGGCAGAGAGCGAGACTTAGATCGGAAGAGCACACGTCTGAACTCCA46460.10520771390385836Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)