FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26125141
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT346209413.251962927204872No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA14410715.51603147328468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC11010534.21453419141355No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC6732022.5768358532495577No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT6053132.3169750547949195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT4620931.7687674872261934TruSeq Adapter, Index 8 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT4498141.721766783957262No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT2999721.1482119847697665No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGTAGATCGGAAGAGCACACG2864711.0965337947841123No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2768321.059638300133959No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT2086620.7987019093983071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1974050.7556131467386147No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAAC1731640.6628251307811124Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1680830.6433764319205014No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1659460.6351965717620432No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAA1614840.6181172381040929Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1446590.5537156718120679Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG1395340.5340985528078107No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCACCAGATCGGAAGAGCACACGTCTG1282470.4908949582319958Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG1259560.48212562757077554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT1200660.459580294705395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT1150260.4402885327968182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAGCGCTGAGAAGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1078030.4126408351250621Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT1043290.39934329923807876No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA926390.35459712925568515Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGGCGGAGCGCTGAGAAGACGGAGATCGGAAGAGCACACGTCTGAACTCC913200.3495483526768334Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA894080.34222973188929395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGA862460.33012644792998436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA784110.30013617916932966Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGAGATCGGAAGAGCACACGT698870.2675086040683953No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT679970.26027419335267893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA655290.25082735438633613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT636460.24361973778438173Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG627220.2400829147678093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG582990.2231528626008181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT579540.22183229556540957Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCTG558580.21380937235898553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCCAGATCGGAAGAGCACACGTCTGA555920.21279119603603286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTAGATCGGAAGAGCACACGTCTGA552100.21132900297073995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT547210.2094572427379435TruSeq Adapter, Index 8 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT539990.20669362128992913No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC507050.19408507689968066No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC480270.18383441452048047No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG477460.18275882223946657No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC472770.18096361661741844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGCGGAGCGCTGAGAAGACGGTCGAAAGATCGGAAGAGCACACGTCTGA468590.17936362525277855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAA459510.17588804592480478Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC451090.17266509681230047No Hit
GGGGCTGTAGCTCAGCTGGTTAGAGCATCGTGTAGATCGGAAGAGCACACG418450.1601713843381745No Hit
CTGGCGGAGCGCTGAGAAGACGGTCGAACAGATCGGAAGAGCACACGTCTG401650.1537407970353155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT380290.14556476460739484No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT352880.13507295520433749Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGGTGGAGCAGTCTGGCAGCTCGTCGAGATCGGAAGAGCACACGTCT350070.13399736292332357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGTGCAGATCGGAAGAGCACACG346490.1326270353909286No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT335560.12844332591353286Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGCGGAGCGCTGAGAAGACGGAGATCGGAAGAGCACACGTCTGAACTCCA333030.12747491008756662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC327530.1253696582919878No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC319830.12242230577817743Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC308380.11803955431283605Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC307280.11761850395372028No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT289550.11083193771088164No Hit
CTGGCGGAGCGCTGAGAAGACGGTCGAGATCGGAAGAGCACACGTCTGAAC276290.10575636701826796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGTAGCTCAGCTGGTTAGAGCACCGAGATCGGAAGAGCACACGTCT275060.1052855561621658Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT272130.10416403111470289TruSeq Adapter, Index 8 (100% over 37bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT268480.10276690946854604No Hit
GCGGAGCGCTGAGAAGACGGTCAGATCGGAAGAGCACACGTCTGAACTCCA262030.10029802327191269Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)