FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9712074
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC2502482.5766689998449355No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT2431822.5039141999947696No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1822561.8765919617169309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1191521.226844029400929No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA437210.4501716111306401Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT379830.3910905126958465No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT348580.35891406922970315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA313240.322526372842711Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT298700.30755531722678386No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC287060.29557023556451484TruSeq Adapter, Index 1 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC286050.2945302929116891No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC221300.2278607020498402No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT208580.21476360250138127No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT200810.20676325159795939Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG159830.16456835069419776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA154440.15901855772515736TruSeq Adapter, Index 1 (100% over 37bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC154310.15888470372033822No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC151230.1557133934523151No Hit
GGATGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT149330.15375706568957362No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT145760.15008122878800143Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC134960.13896104992610228No Hit
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT132010.13592359366289836Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT128300.13210360629459783No Hit
GGCGGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC122910.12655381332555743No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC121190.12478282187718091TruSeq Adapter, Index 1 (100% over 50bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC118650.1221675205522528Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT117590.12107609558988121No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG116070.11951103337968799Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC115210.11862553765549974No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA114320.11770915254558398Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGTAAGAGCACACGTC110490.11376560763437346No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG102600.1056416991880416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTTGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC100560.10354122095857178No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT100080.10304699078693182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT99100.10203793751983356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC97380.10026694607145703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)