FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001839745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059869
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC1280822.5313303565764254No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT1254692.479688703403191No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA235850.46611878686977865Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC195840.3870455934728745TruSeq Adapter, Index 8 (100% over 50bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT193870.38315221204343436Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT149060.2945926070418029No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC132000.2608763191300012No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT110060.21751551275339343No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT104220.20597371196764183TruSeq Adapter, Index 8 (100% over 36bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA91350.18053827085246674Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT80180.15846260051396588TruSeq Adapter, Index 8 (100% over 37bp)
GGATGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT79320.1567629517681189No Hit
GGATGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC78900.15593289075270528No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG75330.1488773721216893Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT69920.13818539570886124No Hit
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA69210.136782197325662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC68520.13541852565748244No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT67340.1330864494713203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT62010.122552579918571No Hit
GGCGGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT61010.12057624416758617No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC59550.11769079397114827No Hit
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG56400.11146533635554595TruSeq Adapter, Index 8 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGTAAGAGCACACGTC55920.11051669519507323No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC54720.1081450922938914No Hit
GGCGGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC53580.10589206953776867No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC52320.10340188649152775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)