FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001839747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24900822
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC22607539.07902959990638TruSeq Adapter, Index 4 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC7519053.0195991120293137No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT5385342.162715752917715No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC2090070.8393578332474326Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2041470.819840405268549Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA1909170.7667096291038104TruSeq Adapter, Index 4 (100% over 40bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1895400.7611796911764599TruSeq Adapter, Index 2 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1618690.6500548455789934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1464260.5880368126000017No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC1440840.5786315005986549Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1392610.5592626620920386Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1391350.5587566546999935Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC1330890.534476331745193TruSeq Adapter, Index 4 (100% over 37bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT1203180.48318886822290447TruSeq Adapter, Index 4 (100% over 41bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1052600.4227169689418285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC1006800.40432400183415634TruSeq Adapter, Index 4 (100% over 38bp)
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT991300.39809930772566465Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC815850.32763978634922175TruSeq Adapter, Index 4 (100% over 38bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA765960.3076043031832443TruSeq Adapter, Index 4 (100% over 39bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA743320.29851223385316356Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT659640.2649069175306743No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC632490.2540036630116066No Hit
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA607420.2439357222825817TruSeq Adapter, Index 4 (100% over 39bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT605500.24316466339946527Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGGGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA570280.22902055201229904TruSeq Adapter, Index 4 (100% over 39bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC564280.2266109930025603TruSeq Adapter, Index 4 (100% over 38bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA506870.2035555292110437TruSeq Adapter, Index 4 (100% over 40bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC499810.20072028144291781TruSeq Adapter, Index 4 (100% over 37bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC452840.1818574503283466TruSeq Adapter, Index 4 (100% over 37bp)
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACTC445430.17888164495131928Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA423670.17014297760933353TruSeq Adapter, Index 4 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC391360.15716750234189056No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC389630.15647274616074924TruSeq Adapter, Index 4 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT386020.1550229948232231No Hit
AGAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC370790.14890673087016967TruSeq Adapter, Index 4 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC359940.1445494449942255No Hit
AAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA326080.13095150031593336TruSeq Adapter, Index 4 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC319420.1282768898151234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA312950.12567858201628845TruSeq Adapter, Index 2 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT300780.12079119315820176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC293110.11771097355741912TruSeq Adapter, Index 4 (100% over 38bp)
GGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC287380.11540984470311863TruSeq Adapter, Index 4 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC283230.11374323305471602No Hit
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG271100.10887190792336092Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT265320.10655069941064596Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC255400.10256689518121129Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)