FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001839749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371249
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC7111219.154799070165847TruSeq Adapter, Index 7 (100% over 50bp)
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC35280.9503055900487275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG22200.5979814087041312TruSeq Adapter, Index 7 (100% over 36bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC17950.4835029858666286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA17290.46572516020245175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCAG14460.38949599864242057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA14350.3865330276983911TruSeq Adapter, Index 7 (100% over 37bp)
AACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA13920.37495050491718496TruSeq Adapter, Index 7 (100% over 40bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC13290.3579807622377434No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC12030.3240412768788603No Hit
AAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTCTGAACT11980.3226944719043014Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC10940.29468092843347726Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA10490.2825596836624476Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT8430.22707131871062278No Hit
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT8210.22114537682256385TruSeq Adapter, Index 7 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC7850.2114483810057401Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAAGATCGGAAGAGCACACGTCTGAACTCCA7750.20875477105662238Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT7440.2004045802143575TruSeq Adapter, Index 7 (100% over 41bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG6990.1882833354433278Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAAATAGGAGAAGATCGGAAGAGCACACGTCTGAACTC6170.16619573386056258Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGAGGAGAAGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA5920.15946170898776832TruSeq Adapter, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC5780.1556906550590035No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT4920.13252560949659126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC4880.13144816551694416TruSeq Adapter, Index 7 (100% over 39bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT4700.12659966760853228Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA4700.12659966760853228TruSeq Adapter, Index 7 (100% over 37bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT4400.11851883776117915TruSeq Adapter, Index 7 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT4080.10989928592400248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT3890.10478142702067883TruSeq Adapter, Index 7 (100% over 38bp)
AAATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTCC3830.10316526105120821Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA3800.1023571780664729TruSeq Adapter, Index 7 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)