FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23416521
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT841924935.9543119150791No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC21108689.01443899373438No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT7640253.2627605099835284No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC6877342.9369606185308226No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT3952441.687885232823441No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC3887951.6603448479814744No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3484411.4880135268599464TruSeq Adapter, Index 4 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT2853751.2186908550591269No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1775550.758246709662806No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT1765930.7541384990537237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACGT1710880.7306294560152637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1507370.6437207303339382No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1497730.6396039787464586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG1265020.5402254246051323No Hit
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC1235440.5275933175555839TruSeq Adapter, Index 4 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC891010.38050485808715995No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT868950.3710841589149814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT864080.3690044306752485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT827180.3532463255323026No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA662890.2830864584880051Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT633620.27058673660361415Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG630830.26939527011719633No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT623870.26642300963494964No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC518770.22154016815734498No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC455580.1945549469112No Hit
TCCCTGGTGGTCTAGTGGTAGGATTCGGCGAGATCGGAAGAGCACACGTCT435850.1861292717223024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG431740.18437410066166532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT396620.16937614259607564No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC380110.162325564929137No Hit
GGCTGGTCCGATGGTAGTGGGTTATCGGAACTAGATCGGAAGAGCACACGT357610.15271696423221878No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT349800.14938171216808852Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGTC322150.13757380953387566No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA319210.13631828570947838TruSeq Adapter, Index 2 (97% over 36bp)
GAGGGCGCGCGGGTCGGGGCGGCAGATCGGAAGAGCACACGTCTGAACTCC313020.1336748528955262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA311010.1328164845666015No Hit
GAGGGCGCGCGGGTCGGGGCGGCGGAGATCGGAAGAGCACACGTCTGAACT310330.13252609130109463Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGCCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT286350.12228545820277914No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT283890.12123491785991608No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC283470.1210555573135736No Hit
GGCTGGTCCGGTGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT281570.12024416436583385No Hit
GAGGGCGCGCGGGTCGGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTC281050.12002209892750507Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGGACTAGATCGGAAGAGCACACGT278930.11911675521739545No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT261190.11154090737902526No Hit
GGCTGGTCCGATGGTAGTGGGTTACCAGAACTAGATCGGAAGAGCACACGT248930.10630528762150449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)