FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25861538
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT385669114.912844703977001No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT12542784.849974506543269TruSeq Adapter, Index 2 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC11584244.479331430327152No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC6153602.3794408515069754No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC5450302.1074926015614386TruSeq Adapter, Index 2 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT3566931.3792412500756916No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT3347871.294536311026823No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT2752211.064209715601601Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC2230680.8625473086712786No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC1726750.6676903747951881No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT1640030.6341579530188808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1466220.5669500398622851No Hit
GGGGCTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1138940.44039917502199605TruSeq Adapter, Index 2 (100% over 37bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1108140.4284895971770898Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT1049330.40574926363621533No Hit
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT964960.3731255271824901TruSeq Adapter, Index 2 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA959660.37107615177411335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGTGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT894680.34595003591820406TruSeq Adapter, Index 2 (100% over 37bp)
CGGGCGGTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG803030.31051130833750107TruSeq Adapter, Index 2 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC753870.2915023847382936No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT661630.2558355191404316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC626680.2423212416833059No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG549740.21257049754736163No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT533900.20644557179855275No Hit
ACGGGCGGTGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA498800.1928732931506239TruSeq Adapter, Index 2 (100% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG469880.1816906635637834Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT439070.16977721897282366TruSeq Adapter, Index 2 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG418480.1618155888485828No Hit
ACGGGCGGTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT416670.16111570781289186TruSeq Adapter, Index 2 (100% over 37bp)
GGGGCTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT411200.15900059772160496TruSeq Adapter, Index 2 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT395300.1528524714964748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT368350.14243159088218188No Hit
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA348880.13490303631593759Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTGGTCCGAGTGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA344550.13322873527475435Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG333270.12886704572636012No Hit
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA330450.12777662333926157TruSeq Adapter, Index 2 (100% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT317110.12261838410383792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT313320.12115288734954588TruSeq Adapter, Index 2 (100% over 37bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG294430.11384860405440698TruSeq Adapter, Index 2 (100% over 38bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC262920.10166448723969936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)