FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21427416
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT18831928.788703220210966No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC9342564.360096429732825TruSeq Adapter, Index 3 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC7185133.3532414734469147No Hit
ACGGCCCTGGCGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT5325692.4854560157883716Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2649621.236556008433308No Hit
ACGGCCCTGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA2000890.9337990171096692TruSeq Adapter, Index 3 (100% over 36bp)
ACGGCCCTGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1952510.9112204663408785TruSeq Adapter, Index 3 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT1775920.8288073559593No Hit
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1331290.6213021672795264TruSeq Adapter, Index 3 (100% over 37bp)
AGAAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT745610.3479700958809032Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG684650.31952056188203004TruSeq Adapter, Index 3 (100% over 37bp)
AGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG642250.29973282826076647TruSeq Adapter, Index 3 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT617400.2881355362681156No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC582080.2716519808081385No Hit
ACGGCCCTGGCGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT578200.26984121650506065Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA490090.2287209993029491Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGCCCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG474800.22158528121169627TruSeq Adapter, Index 3 (100% over 38bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG402020.18761944977406514Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT394040.18389524896515755No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC381220.17791225969570945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA363500.1696424804558795TruSeq Adapter, Index 3 (100% over 36bp)
AGAGGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC352420.16447153497183234TruSeq Adapter, Index 3 (100% over 39bp)
ACGGCCCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC328930.15350894386892008TruSeq Adapter, Index 3 (100% over 39bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC326870.15254755869769832Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT319700.14920137827164975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC310820.14505715481512096No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT298790.13944285209191815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC296400.1383274586165686TruSeq Adapter, Index 3 (100% over 39bp)
AGGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG292090.13631601682629393TruSeq Adapter, Index 3 (100% over 38bp)
AGGGGCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG274360.12804157066815708TruSeq Adapter, Index 3 (100% over 37bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG263200.12283328983765471TruSeq Adapter, Index 3 (100% over 37bp)
AAGCCGGCGGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA262860.12267461461522006TruSeq Adapter, Index 3 (100% over 36bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC246760.11516087614110819No Hit
AAATGGATTTTTGGAGCAGGGAGAAGATCGGAAGAGCACACGTCTGAACTC246690.1151282077129599Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCT236530.11038661871314767TruSeq Adapter, Index 3 (100% over 51bp)
AGAGGGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG227640.106237728338312TruSeq Adapter, Index 3 (100% over 38bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG218220.10184149129321055TruSeq Adapter, Index 3 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)