FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17196322
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC182724310.625778000667816No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT15881159.235201573917957No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT12983077.549910963518827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2997921.743349537185917No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT2835261.6487595428836468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT2635541.532618428522099No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2084611.212241780538885No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT1961321.1405462168014766No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG1690640.9831404645714357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA1325000.7705136016876166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1219940.709419142070031TruSeq Adapter, Index 7 (100% over 37bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT1127500.6556634610587078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC931740.541825164706732No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC889880.5174827500903972No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTC799070.4646749461890747No Hit
GGGGCCGTAGCTCAGCTGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT796240.4630292454398098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCGTAGCTCAGCTGGGAGAGCGCTGCAATAGATCGGAAGAGCACACG784070.4559521506982714No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAGATCGGAAGAGCACACGTCT765370.4450777323197368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC764400.4445136582113315No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATAGATCGGAAGAGCACACG737110.4286439856150635No Hit
CGCGGGGTGGAGCAGCCCGGTAGCTCGTCAGGCTAGATCGGAAGAGCACAC594060.34545759261777026No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAAGATCGGAAGAGCACACGTC530390.30843223335780756No Hit
GGGGCCGTAGCTCAGCTGGGAGAGCGCTGCAAGATCGGAAGAGCACACGTC517750.30108182435755737No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG500370.2909750119822134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG499310.2903586011008633No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT496770.28888154106442065No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT486120.28268835626595035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC468950.2727036630274776No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT462710.2690749801033035No Hit
AGGGATATAGTTCAGTTGGAAGAATAGATCGGAAGAGCACACGTCTGAACT437070.25416481501102384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT431190.25074547917862905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGCCGTGGCGGAATTGGTAGACGCGCAACGAGATCGGAAGAGCACACGT417170.24259257299322495No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT392770.22840349232818505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG364750.21210931035136465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT353040.20529971467154431Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT327280.19031976721533825No Hit
AGGAGTGTAGCTCAGTTGGTAGAGCATCGGTAGATCGGAAGAGCACACGTC326570.18990688822877358No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT292680.17019918561655217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC291460.1694897315833002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT276900.1610228047602272Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT247100.14369351771849817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC215560.12535238639983598No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC213350.12406722786419096TruSeq Adapter, Index 7 (100% over 50bp)
GCGGCCGTGGCGGAATTGGTAGACGCGCAACAGATCGGAAGAGCACACGTC211450.1229623404353559No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG209970.12210169128026331No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT206060.11982794925566059Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCCGTAGCTCAGCTGGGAGAGCAGATCGGAAGAGCACACGTCTGAACT199920.11625741830142515Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGTC192450.11191346614700516No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGAA185450.107842828251297Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT184650.10737761249178748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGTC180690.10507479448221545No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGT173200.1007192119338077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)