FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001839765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001839765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19298194
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTC236458712.252892680009332No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT13271566.877099484024256No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT9691345.02188961309022Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT2295531.189505090476342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACGT2279281.1810846134099389No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1950851.0108977036918585No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGTC1278590.6625438629127679No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG1244980.6451277254234256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT1093690.5667317884772015Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT965400.5002540652249635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT886880.4595663200401032TruSeq Adapter, Index 2 (100% over 37bp)
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT802340.41575911196664306TruSeq Adapter, Index 2 (100% over 37bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGAGATCGGAAGAGCACACGTCTGAAC784830.4066857240630911Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC623830.3232582282051885No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACGT560620.29050386787489024No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC544040.2819123903511386TruSeq Adapter, Index 2 (100% over 50bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG473880.24555665675244012Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA467450.2422247387501649Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT442120.22909915819065763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT429470.2225441406589653No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGTC421830.2185852209797456No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT397680.20607109660106016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCCA395040.2047030929422722Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTCC384380.1991792599867117Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCAG348140.18040030067062235Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCTG295670.15321122795221148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC282800.14654221011562016No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT270020.1399198287673966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGAGGGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG253620.13142162422038042TruSeq Adapter, Index 2 (100% over 38bp)
GCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT253020.13111071429792862Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT244130.12650406561360095TruSeq Adapter, Index 2 (100% over 39bp)
TCACCGGGTGTAAATCAGCTTGAGATCGGAAGAGCACACGTCTGAACTCCA239280.12399087707378213Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT238370.12351933035806356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAACTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA230580.11948268319823087TruSeq Adapter, Index 2 (100% over 36bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT229360.11885049968924552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC226820.11753431435086618Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA223010.1155600363432972Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACT217790.11285512001796645Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG215780.11181357177775288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACACG214600.111202115596931No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGTCT207160.10734683255852852Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGAGATCGGAAGAGCACACGTCTGA205070.10626382966198807Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGGGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG199380.10331536723073673TruSeq Adapter, Index 2 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)